Introduction Identifying blood samples is a crucial step of a forensic investigation, especially of major and violent crimes. Animal and human blood look alike to a naked eye but its biochemical aspects are different.It is extremely important to analyse whether evidentiary blood stains are of human origin or not in a forensic investigation. Thus differentiating blood samples is important in a forensic investigation. Aim To differentiate human blood from animal blood in the given blood samples for forensic purposes. Materials and methods Blood samples of each human and animal (Wistar albino rat) was collected from clinical lab and animal house respectively.The difference in the constituents of blood and various cell counts was analysed with the help of a simple microscope and CBC (complete cell count) machine. Statistical analysis was done using SPSS software version 23.0 and corresponding graphs were plotted. Results A significant difference was observed in the cell counts and blood group type.The platelet and RBC count of that of wistar albino rats was higher than humans. There were similar haemoglobin levels. The animal blood sample taken did not fall under the ABO blood grouping unlike that of the humans. Conclusion These differences observed are helpful in the differentiation of human and animal blood for forensic purposes. Usage of advanced technologies will make this differentiation even more easier.

Innovative technique, Blood, Electron microscope, Forensics, Haemoglobin, Hemocytometer, Wistar albino rat, novel method.