Introduction: Hyaluronic acid, as a natural linear polysaccharide, has attracted researchers’ attention from its initial detection and isolation from tissues in 1934 until the present day. Hyaluronic acid has a wide range of uses in bioengineering and biomedicine because of its biocompatibility and high biodegradation rate, from polymeric scaffolds and wound dressings to endoprostheses for joint fluid and biorevitalizing skin cosmetics. The aim of the study was to find about fabrication and Physicochemical characterization of medium molecular weight hyaluronic acid /CuO GBR membrane. Materials and methods: Fabrication of Medium molecular hyaluronic acid, gelatin and Carrageenan and preparation of scaffold of control and test group. Micrographs of all scaffolds were taken at 100X.The expected pendant functionalities of scaffolds were confirmed by the FTIR spectrum. To determine the hydrophilicity of the scaffolds, water contact angles of the scaffolds were measured by goniometer software. Three measurements at different positions of each scaffold were conducted. Swelling/shrinkage studies were performed to calculate the water content (%) of the scaffolds. After obtaining informed consent and ethical approval from SIMATS ethics committee, the Dental Pulp stem cells were isolated from molars. The reaction product was transferred to a 96 well ELISA plate and A590 was measured with ELISA plate reader. All values are expressed as the mean ± standard error of the mean (SEM) of at least three independent experiments. A one-way ANOVA followed by Scheffe’s method. Statistical significance was set at p < 0.05. Results: The contact angle shows hydrophilic nature in addition with CuO nanoparticles and also elevation of swelling indicates it can be used in the chemo static based membrane or socket preservation procedures. The addition in CuO decrease in porosity is highly evident that CuO based particles have higher cell adherence, increased cell migration, increased cell penetrating efficiency and a high flow of nutrition. Conclusion: In this study, it has been concluded that a new material made by a medium molecular weight/CuO hybrid was fabricated and tested using Dental Pulp stem cells. We found that M-HLA exhibited a stronger osteoregenerative and better osteoconductive properties and significant intracellular responses for bone grafts, making it reasonable to suggest this M-HLA/CuO hybrid as a potential novel dental material for guided bone regeneration surgery.

biocompatibility, degradability, hyaluronic acid, molecular weight, structure, receptors, viscosity, water polymer solution