Volume 14 No 1 (2022): International Journal of Early Childhood Special Education

Efficacy Of WrighteriaTinctoria Oil As A Natural Alternative To DPX Mounting Media


Aswani.E

DOI: 10.9756/INT-JECSE/V14I1.388

Keywords: Wrighteriatinctoria oil, clove oil, castor oil, DPX, Mounting media, refractive index


Abstract

Background: Mounting is a series of histological slide preparation. Most commonly used mountant is DPX, but owing to its hazardous nature, organic alternatives should be sought for. The aim of the present study is to evaluate and compare the efficacy of wrighteriatinctoria oil (WT oil) as an alternative to gold standard DPX mounting media Material and methods: 19 histopathologically confirmed cases of OKC blocks were retrieved from the Department of Oral Pathology and four section were made from each block. Sections were subjected to routine H& E staining and four different mountants and a cytological assessment was made. Kruskal – Wallis ANOVA, was done to compare the groups. p< 0.05 was considered to be statistically significant. Result: WT oil and DPX(2.63) had similar clarity of cellular details. Based on the adhesion DPX had a maximum median score of 2.95 compared to other natural alternatives. Conclusion: WT oil showed superior quality as a mountant over castor and clove oil. It can be used as an alternative temporary mounting medium to DPX with the addition of some adhesive agents because of its excellent refractive index.

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Volume 14 No 1 (2022): International Journal of Early Childhood Special Education

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Research Articles

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